Application of Nanocoulter I in Viral Vectors

I. Characteristics of Commonly-Used Viral Vectors

II. Application of Nanocoulter I after Viral Vector Purification

III. High-precision and Ultra-sensitive Detection Platform in the Fields of Bio-nano Drugs and Nanomaterials

1. Main characteristics of Nanocoulter Ⅰ:

• Nanoparticles in a single detection fluid

• Disposable detection card to avoid cross contamination

• Multidimensional data for visualization during test

• It can automatically analyze the particle size distribution, concentration and potential data of nanoparticles without benchmarking

• Any type of nanoparticles (inorganic & organic, transparent & opaque, conductive & insulating)

• Measurement range of particle size: 40-2000 nm

• Concentration measurement range: 106-1012 / ml

• Detection speed: The test is completed within 5 minutes

• Sample demand quantity: Within 50 μl

• Desktop size, 400 (mm)×350 (mm)×310 (mm)

• Weight: 19 kg

2. Technical principle of Nanocoulter Ⅰ:

Coulter Principle (also known as: resistive pulse sensing method or RPS): The particles suspended in the electrolyte replace the same volume of electrolyte as the electrolyte passes through the small hole, resulting in a transient change in resistance between the two electrodes inside and outside the small hole tube in a circuit designed for constant current, generating a potential pulse. The size and number of pulse signals are proportional to the size and number of particles. Coulter Principle belongs to the measurement of individual particles and three-dimensional measurement. It can not only accurately measure the particle size distribution of materials, but also measure the ** number and concentration of particles. The measured particle size is closer to the real one and is not affected by the physical and chemical properties of the sample.

3. Nanocoulter Ⅰ lentivirus test:

Test steps:

①The two samples including PC (before filtering) and P02 (after filtering) are used to characterize the difference between the samples before and after filtering.

②The 2 samples were diluted with 004 diluent and tested to see the distribution of large particles.

Fig. 1 (Exosome sample test data)

Fig. 2 (Experiment test report)

Test summary:
The concentration of large particles in exosome sample is around 4.77E+10, and the membrane filtration results in a loss of about 60% of the particles. Moreover, the concentration is around 1.65E+10, with a decrease in large particles and a corresponding decrease in average particle size.

Single particle detection is performed to obtain the true particle size distribution of the samples, which can accurately characterize the difference before and after the sample is filtered through membrane filtration.